The field of gene editing has rapidly advanced from blunt genetic disruptions to precision-controlled genomic modifications. Among the most powerful applications of this evolution is the creation of knock-in (KI) cell lines, which allow researchers to insert custom DNA elements—such as reporter genes, tags, or regulatory sequences—into a precise location within the genome.
These genetically modified cell lines are invaluable tools in modern biology. They offer direct insight into gene function, enable real-time observation of cellular processes, and form the backbone of many high-throughput drug screening platforms.
This article introduces the key concepts behind knock-in cell line construction, discusses the most common strategies, and explores their broad applications in biomedical research.
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Colorectal cancer (CRC) remains one of the leading causes of cancer-related deaths worldwide. As research into the molecular mechanisms of this disease deepens, the demand for reliable, genetically representative in vitro models grows. Among these, the SW480 cell line has earned recognition as one of the most widely used human colon cancer models. First established from a 50-year-old male patient with primary adenocarcinoma of the rectum, SW480 cells provide researchers with a versatile platform for exploring tumor biology, drug response, and gene function.
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How Scientists Build Point Mutation Cell Lines: An Overview of Current Strategies
In modern biomedical research, the ability to model human disease at the cellular level has become increasingly crucial. With the advent of next-generation sequencing (NGS), thousands of disease-associated single nucleotide polymorphisms (SNPs) have been identified. To explore the biological consequences of these variants, researchers often rely on point mutation cell line construction to introduce precise, predefined mutations into the genome.
For researchers looking to build such models, there are several gene editing strategies to consider. Each has its own benefits and challenges, and the best choice often depends on the target site, mutation type, and available resources.
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Gene editing has undergone a remarkable transformation over the past decade, with technologies like CRISPR-Cas9 paving the way for groundbreaking advancements. Among the latest innovations is prime editing, a technique that offers unprecedented precision in modifying genetic sequences. For researchers and clinicians aiming to correct genetic mutations with minimal off-target effects, prime editing presents a promising solution.
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CRISPR/Cas9 technology has revolutionized the way researchers investigate gene function. Creating knockout (KO) cell lines—cells where a specific gene is entirely disabled—has become an essential approach in gene function analysis, drug development, and disease modeling. However, generating a stable KO cell line can be complex, often requiring both strategic planning and technical finesse.
If you're wondering how to make KO cells effectively, this article breaks down the essential steps and shares insights from hands-on experience in the field.
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