The field of gene editing has rapidly advanced from blunt genetic disruptions to precision-controlled genomic modifications. Among the most powerful applications of this evolution is the creation of knock-in (KI) cell lines, which allow researchers to insert custom DNA elements—such as reporter genes, tags, or regulatory sequences—into a precise location within the genome.
These genetically modified cell lines are invaluable tools in modern biology. They offer direct insight into gene function, enable real-time observation of cellular processes, and form the backbone of many high-throughput drug screening platforms.
This article introduces the key concepts behind knock-in cell line construction, discusses the most common strategies, and explores their broad applications in biomedical research.
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