CRISPR/Cas9 technology has revolutionized the way researchers investigate gene function. Creating knockout (KO) cell lines—cells where a specific gene is entirely disabled—has become an essential approach in gene function analysis, drug development, and disease modeling. However, generating a stable KO cell line can be complex, often requiring both strategic planning and technical finesse.
If you're wondering how to make KO cells effectively, this article breaks down the essential steps and shares insights from hands-on experience in the field.
https://www.ubigene.us/app...