The baculovirus expression vector system (BEVS) is a powerful tool to produce recombinant protein on both laboratory and industrial scales for various applications such as production of vaccines and pharmaceutical proteins. It displays several advantages over other expression systems, including the capacity for high expression levels, large DNA insertion, and post-translational modifications of numerous proteins similar to those in mammalian cells. To improve the efficiency of protein production, optimization of a series of parameters is required. Among them, the ratio of input virus to the number of cells in the culture, also called the multiplicity of infection (MOI), is one of the most important factors. Therefore, accurate measurement of virus titer before protein production is much essential.

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The baculovirus expression vector system (BEVS), first described in the 1980s, has proven successful for the production of many recombinant proteins. In principle, any foreign gene may be expressed in the BEVS for producing proteins in insect cells (IC). Protein therapeutics such as enzymes, tissue plasminogen activator (tPA), and viral and parasitic proteins have been produced using the BEVS-IC. Some viral structural proteins have been proven to self-assemble into ordered structures such as virus-like particles (VLP) during production in BEVS. These virus-like particles are commonly remarkably immunogenic and therefore potentially useful as vaccines. The baculovirus expression system is also promising for delivering genes into mammalian cells for gene therapy and other diverse applications.

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Adenoviral vectors continue to be one of the preferential vehicles for gene therapy because they have many advantages over other vectors. They have a high packaging capacity and can accommodate large transgene inserts of sizes up to ~8 kb. Adenovirus vectors also have high transduction efficiency and they can infect both dividing and non-dividing cells in many cell and tissue types. Compared with e.g. lentiviruses, adenovirus DNA does not integrate into the host genome but remains episomal. Besides, adenovirus vectors can be produced in high titers and the particles are stable yet easy to modify. At present, a number of gene therapy clinical trials are ongoing with adenoviral vectors. Most of these trials are for the treatment of cancer, although some are for use of adenoviral vectors as vaccines in which the vector expresses a foreign antigenic protein or for gene therapy in which the vector expresses a non-mutant protein to correct a genetic defect.

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Creative Bioarray has deep expertise in the profiling, identification and structural characterization of metabolites in vitro and in vivo. We use advanced technology and software for accurate, reliable metabolite identification and structural characterization. In addition, synthetic chemistry services are available to undertake synthesis of metabolites, reference standards, and internal standards to aid in method development and metabolite identification.

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Creative Biogene is a biotechnology company specializing in custom miRNA microarray service. Creative Biogene's sophisticated equipments, advanced technologies and highly experienced staffs are available to assist you with the completely customized miRNA microarrays. Creative Biogene's top priorities are to streamline the platform processes or work with you to design a microarray that meets your specific needs. Creative Biogene is confident in providing you with the most affordable, high-quality custom miRNA microarray service in a short turnaround time.

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As a pioneer and leader in the drug discovery service, Creative Biogene has a high level of proficiency in providing Heat Shock Protein 90 (HSP90) Screening and Profiling Services for customers worldwide. Creative Biogene, which has experts on HSP90 screening, together with Our innovative technology platforms can offer you with high-quality, comprehensive service with the most competitive price.

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Creative Biogene, a leading biotechnology service vendor in the world, has years of professional and abundant experience which are available to provide you a full spectrum of high quality baculovirus services ranging from baculovirus transfer vector construction, recombinant bacmid DNA preparation, baculovirus production as well as baculovirus titer assay.

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CRISPR/Cas9 PlatformCB offers a one-stop plant genome editing service, including gene knockout, gene knockin and gene deletion. Various species are available for this service, including but not limited to Arabidopsis thaliana, Zea mays, rice and tobacco. With excellent platform, our experienced scientists are dedicated to providing a reliable and satisfied service for our customers.

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CRISPR/Cas9 PlatformCB provides a knockin mice generation service, including point mutation mice, ROSA26 knockin mice, custom knockin mice and conditional knockin mice. With years of experience and expertise, our staff will show you a reliable strategy for your case after analysis the situation. We will work closely with you to design and deliver a genetically engineered mouse model to your exact specifications.

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As a leading provider of gene editing service, CRISPR/Cas9 PlatformCB is proud to offer gene knockout cell line generation service with high quality. Depending on the excellent platform and experienced scientists, our staff has succeeded in lots of gene knockout projects, ranging from tumor cell line to difficult-to-handle cell line. At CRISPR/Cas9 PlatformCB, we take the time to understand every case to help ensure you receive the edited cell line you desire. Our scientists will work with you closely to assist you in achieving your research goal.

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IntegrateRNA has devoted to improve the circRNA overexpression technology and explore related products for many years, to support the functional research of circRNA. We provide one-stop solution for circRNA overexpression, including vector construction, virus packaging (optional), cell transfection and qPCR validation. Our excellent service will facilitate your research project and help you explore the potential clinical applications of circRNA.

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Circular RNAs (circRNAs) are covalently closed RNA molecules generated mainly from pre-mRNA back-splicing, and have also been found to function as mRNAs to direct protein synthesis inside cells. As a new generation of therapeutic mRNAs, circRNAs may address some of the limitations of linear mRNA including increased stability, reduced immunogenicity, and tissue/cell-specific translation. Therefore, translatable circRNAs are able to produce more therapeutic protein inside the body than conventional mRNA, with the potential to engineer cells, replace damaged proteins, or add new beneficial proteins for patients with serious diseases.

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LncRNA qPCR assay offered by IntegrateRNA is designed for SYBR Green based, real-time PCR detection of long non-coding RNAs. Our service is available for almost all human, mouse and rat long non-coding genes annotated by NCBI and GENCODE. We utilize qPCR assay to help you validate selected lncRNAs from microarray profiling data and RNA-seq data. We are confident to manage the entire lncRNA qPCR workflow and deliver reliable data in a timely and cost-effective manner.

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Microbial genomes usually contain thousands of genes. It is particularly important to discover the correlation between these genes and phenotypes with high throughput and low cost. Identification of gene function has been aided by the ability to generate targeted gene knockouts or transcriptional repression using the CRISPR/Cas9 system. Inhibiting or activating gene expression by the genome-wide CRISPR library can help researchers analyze gene groups associated with a certain phenotype at the whole-genome level under a variety of screening conditions, providing more valuable discoveries for microbial cell factory research.

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Creative Biogene can use CRISPR/Cas9 gene editing, T-DNA insertion, homologous recombination, RNA interference and other technologies to assist scientific researchers in exploring gene functions and modifying fungal genomes. Various fungi have differences in the efficiencies of transformation, expression systems, and gene editing. After years of technology accumulation, transformation and genome modification have been successfully achieved in fungi, such as yeasts (e.g. Pichia pastoris, Saccharomyces cerevisiae), Candida albicans, Talaromyces marneffei, Monascus, Aspergillus niger, Penicillium, Rhizopus, Mucor. We can also provide customized genome editing services for other fungi according to clients' requirements.

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