Creative Biogene, a zebrafish research company with experienced scientists and advanced technology, can offer multiple gene-editing services including but not limited to point mutations, knockout, short insertions, gene knock-in and conditional knock-in. We use CRISPR-Cas9 (Tol2 available) as our genome editing methods. Our service can significantly reduce your experiment time and can be tailored to different experimental requirements.

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Creative Biogene can utilize zebrafish models to help you explore reliable detection parameters for drug-induced cardiotoxicity evaluation. Our services contribute to improving the understanding of drug-induced cardiotoxicity, and providing an easy in vivo model for screening drug candidates for cardiotoxicity in the early phase of drug development.

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Visual impairment is a major health problem affecting millions of people around the world. Major causes of visual impairment and blindness include cataract, glaucoma, age-related macular degeneration (AMD), and diabetic retinopathy (DR), with cataracts being the leading cause of blindness worldwide. Strategies to develop animal models that closely mirror human ocular diseases are necessary for a better understanding of the underlying causes and simultaneously for the development of novel therapeutic approaches. Recently, zebrafish have been at the leading edge of preclinical therapy development, with their amenability to genetic manipulation facilitating the generation of robust ocular disease models required for large-scale genetic and drug screening programs.

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In microbial DNA sequencing, because of the large number of species, traditional sequencing methods cannot be used to solve the microbial problems. Creative Biogene has many sequencing methods based on a professional high-throughput platform, and provides you with a full range of microbial DNA sequencing solutions.

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Antibiotics are critical for the treatment of infectious diseases in humans and are often used agriculturally to control the spread of disease among livestock and crops. Antibiotic production can be grouped into three methods: natural fermentation, semi-synthetic, and synthetic. Semi-synthetic production of antibiotics is a combination of natural fermentation and laboratory work to maximize the antibiotic. Antibiotics are produced industrially by fermentation process, where the source microorganism is grown in large fermenters containing a liquid growth medium. Membrane filtration is also used in the production of antibiotics.

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Creative Biogene offers custom microbial expression and production services with cutting-edge solutions. Now customers can flexibly choose an option that fits their requirements, from codon optimization to gene synthesis to small-scale testing and scale-up, together with protein purification and characterization. Our customized workflow can meet your downstream applications.

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QVirus™ Platform offers a comprehensive portfolio of oncolytic VACV services for the efficient design, development, manufacturing and analytical testing of virus vector products. So far, we have developed an efficient way to generate recombinant VACVs, including inserting and/or deleting GOI(s) in VACVs. We also provide customized oncolytic vaccinia virus and proof-of-concept validation study for the oncolytic virus.

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The CRISPR/Cas9 system is widely used for various genome editing approaches in cultured cells and living organisms and was broadly explored for preclinical applications. This two component system is composed of the Cas9 endonuclease that acts in cooperation with a chimeric guide RNA (gRNA) mediating the sequence-specific binding to its complementary target protospacer sequence preceding a protospacer adjunct motif (PAM). Because of its simple gRNA design and easy cloning procedure for customization, the CRISPR/Cas9 system is easier to handle than transcription activator-like effector nucleases (TALENs) and artificial zinc finger nucleases (ZFN). As veritable gene delivery vehicles, viral vectors could be particularly fit to broaden the applicability of RGNs to other cell types including dividing and quiescent primary cells.

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Adenovirus is a replication-defective adenoviral vector system that widely used for gene delivery in a variety of cell types. The vectors are predominantly nonenveloped, nonintegrating, episomal, double-stranded DNA viruses. With a strong safety record, adenoviruses and recombinant adenoviral vectors have been widely used for gene function studies and human gene therapy.

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Creative Biogene is a leading biotechnology company offering the best Pseudomonas genome editing services. With years of experience and expertise in microbial genome editing, our talented scientists will work closely with you to provide any help in Pseudomonas genome editing services.

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Yeast fermentation is the most economical eukaryotic expression system for scale up production of both secreted and intracellular proteins that require post-translational modifications, including humanized antibodies. Yeast fermentation has been also used in the production of chemical precursors, global food processing such as coffee and chocolate, or even wastewater processing.

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Creative Biogene is a leading company offering the best microbial gene expression service. Backed by years of development and research experience, our talented scientists will help you achieve your research goals quickly and efficiently. They would research closely with you to offer your one stop service in microbial gene expression system. All you need to do is simply provide the gene sequences.

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The baculovirus expression vector system (BEVS) is a powerful tool to produce recombinant protein on both laboratory and industrial scales for various applications such as production of vaccines and pharmaceutical proteins. It displays several advantages over other expression systems, including the capacity for high expression levels, large DNA insertion, and post-translational modifications of numerous proteins similar to those in mammalian cells. To improve the efficiency of protein production, optimization of a series of parameters is required. Among them, the ratio of input virus to the number of cells in the culture, also called the multiplicity of infection (MOI), is one of the most important factors. Therefore, accurate measurement of virus titer before protein production is much essential.

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The baculovirus expression vector system (BEVS), first described in the 1980s, has proven successful for the production of many recombinant proteins. In principle, any foreign gene may be expressed in the BEVS for producing proteins in insect cells (IC). Protein therapeutics such as enzymes, tissue plasminogen activator (tPA), and viral and parasitic proteins have been produced using the BEVS-IC. Some viral structural proteins have been proven to self-assemble into ordered structures such as virus-like particles (VLP) during production in BEVS. These virus-like particles are commonly remarkably immunogenic and therefore potentially useful as vaccines. The baculovirus expression system is also promising for delivering genes into mammalian cells for gene therapy and other diverse applications.

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Adenoviral vectors continue to be one of the preferential vehicles for gene therapy because they have many advantages over other vectors. They have a high packaging capacity and can accommodate large transgene inserts of sizes up to ~8 kb. Adenovirus vectors also have high transduction efficiency and they can infect both dividing and non-dividing cells in many cell and tissue types. Compared with e.g. lentiviruses, adenovirus DNA does not integrate into the host genome but remains episomal. Besides, adenovirus vectors can be produced in high titers and the particles are stable yet easy to modify. At present, a number of gene therapy clinical trials are ongoing with adenoviral vectors. Most of these trials are for the treatment of cancer, although some are for use of adenoviral vectors as vaccines in which the vector expresses a foreign antigenic protein or for gene therapy in which the vector expresses a non-mutant protein to correct a genetic defect.

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