The T84 cell line is a transplantable human cancer cell line derived from a lung metastasis of colon cancer in a 72-year-old male. T84 cells spontaneously differentiate into a structurally and functionally mature absorptive epithelial monolayer upon confluence. This maturation is complete over a period of 2-3 weeks and monolayer integrity is routinely assessed by measuring transepithelial electrical resistance (TEER).

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