Engineered using CRISPR technology, these cell lines are rigorously verified via PCR and sequencing to confirm the double-knockout genotype. The resulting knockout cell lysate, prepared in RIPA buffer, includes a parental cell line lysate for internal control. Primarily serving as negative controls for western blots, these lysates validate antibody specificity, crucial for eliminating nonspecific binding. With potential applications as negative controls for functional assays, Creative Biogene's Knockout Cell Lysate series offers researchers a reliable solution for precise genotype confirmation and experimental validation.
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7 months ago