Cell co-culture is a cell cultivation set-up, in which two or more types of cells are cultured in one system. The co-culture of different cells can induce cell differentiation, maintain cell function and viability, and regulate cell proliferation. Conventional monolayer cell culture may gradually lose the original biological characteristics due to the environment change in vitro. Cell co-culture, which is developed to solve this problem, can be used to mimic the microenvironment in vivo. This system is quite useful to study natural or synthetic interactions between cell populations and understand the cellular internal mechanisms.

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