In IEF, proteins migrate to their respective pI and remain stabilized. To conduct successful IEF, stable and linear pH gradients are important. Creative Proteomics provides a high-resolution IEF capable of separating proteins with different pI point. Depending on your samples, different antibodies, antigens, and enzymes will be employed to ensure the successful fractionation. Buffers and ampholyte conditions will also be optimized to separate proteins across a broad range of pH. https://www.creative-prote...