Each restriction enzyme has optimal reaction (assay) conditions and different conditions for long term storage. The recommended assay and storage conditions are determined by the manufacturer in order to provide the user with the highest activity, best fidelity and maximum stability for each enzyme. Factors that must be considered include temperature, pH, enzyme cofactor, salt composition, ionic strength and stabilizer. Setting up a digestion using a single restriction enzyme is relatively simple. However, digestions using multiple enzymes with different buffer requirements may require the use of alternative buffers and may require adjustment of the number of units of enzyme used. Creative Enzymes offer a one-stop standard restriction enzyme reaction service that can meet your research needs for restriction enzyme research. https://molecular-tools.cr...