One helpful technique for synthesizing and semi-synthetic protein synthesis is native chemical ligation (NCL). The ability to glycosylate a synthetic component of NCL site-specifically while maintaining its natural sugar-protein connections excites us. The assembly of glycoproteins that are not easily obtained from biological sources is made possible by this method.

It is convenient to employ NCL for the synthesis of active proteins from smaller fragments because it involves the reaction of a C-terminal peptide thioester with an N-terminal cysteinyl peptide to form a native peptide bond between the two fragments. This method enables the regulated introduction of various protein modifications, including as glycosylation, phosphorylation, acetylation, methylation, and sulfation, into smaller peptide fragments that are suitable to complete chemical synthesis.
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