μWB is utilized to examine the human serum (HIV immunoreactivity) and cell lysate (NFκB) in order to validate the microfluidic chip-based test. A short duration of 10~60 min, multiple analyte detection, mass sensitivity at the femtogram level, high-sensitivity limits (50-pM), and quantitation capabilities over a 3.6-log scope are among the analytical performance improvements. A polyacrylamide gel that is both photopatterned and photoreactive is essential to the multistep test design. The hydrophilic polymer creates a protein immobilization scaffold for subsequent antibody probing of immobilized protein bands as well as a separation matrix for protein size following brief light exposure. Under sizing circumstances, the protein capture efficiency is greater than 75%.
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