Downstream Processing: Essential Steps for Biopharmaceutical Manufacturing

Purification is one of the most important steps in downstream processing as it separates the product of interest from other contaminants in the fermentation or cell culture broth. There are several unit operations used for purification including centrifugation, flocculation, micro/ultrafiltration, and chromatography.

Centrifugation employs centrifugal force to separate cell debris and insoluble particles from the bulk protein solution. This "clarification" step is usually the first purification step to remove large biomatter before further processing.

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