Due to the stochastic nature of spontaneously differentiating hESC, the use of certain factors is important to direct hESC differentiation specifically to neural lineage. Once the NP cells are generated, the expression of SOX2 will be maintained and other neuroepithelial markers are also beginning to express, such as SOX1, SOX3, Nestin, PSA-NCAM, and MUSASHI-1. What's more, the formation of neural rosettes is the morphologic marker of hESC differentiation to neural cells. Learn more, https://neurost.creative-b...