The baculovirus expression vector system (BEVS) is a powerful tool to produce recombinant protein on both laboratory and industrial scales for various applications such as production of vaccines and pharmaceutical proteins. It displays several advantages over other expression systems, including the capacity for high expression levels, large DNA insertion, and post-translational modifications of numerous proteins similar to those in mammalian cells. To improve the efficiency of protein production, optimization of a series of parameters is required. Among them, the ratio of input virus to the number of cells in the culture, also called the multiplicity of infection (MOI), is one of the most important factors. Therefore, accurate measurement of virus titer before protein production is much essential.

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