High-performance liquid chromatography (HPLC) columns are used for the analysis or preparation of separated compounds from complex mixtures. The pressurized liquid solvent containing the sample passes through a column filled with adsorbent material, with components interacting differently and being separated based on flow rates. HPLC column types include normal/reversed phase, mixed-mode, ion exchange, ligand exchange, and affinity column formats. The selection of a column is based on the physical properties of the target component, such as molecular size, hydrophobicity/hydrophilicity, and intermolecular/intramolecular forces.

Amerigo Scientific's HPLC columns can be found at https://www.amerigoscienti...