Cryo Electron Microscopy: A Powerful Technique for Molecular Structure Determination

Cryo electron microscopy (cryo-EM or cryo-TEM) is a powerful technique used to determine the structure of biomolecules at near-atomic resolution. In cryo-EM, biological samples such as proteins, nucleic acids, and protein complexes are rapidly frozen in a thin film of vitreous ice and imaged using an electron microscope. Cryo-EM overcomes many of the limitations of X-ray crystallography by not requiring crystallization of samples. This allows structure determination of more heterogeneous and dynamic molecules.

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