Top 3 Benefits of Dulbecco Medium in Biomedical Research

In vitro experiments with cells require the right culture medium for successful experimentation in biomedical research. One such culture medium that is commonly used is Dulbecco medium.These media are useful in a variety of applications in cell culture, from basic research to therapeutic development, and thus desirable in biomedical research. Let’s take a closer look at the top three benefits of Dulbecco Medium in biomedical research. Read More: https://www.purmabiologics...

The Role of EDTA and Trypsin in Isolating Single-Cell Suspensions

Isolation of single-cell suspensions holds great value in biological and medical research, especially regarding cell analysis, drug testing, and tissue engineering. This involves detaching single cells from a culture surface and separating them from each other. EDTA and trypsin are the two critical reagents required to perform this task. Here, a blog will analyze these two reagents and how they facilitate cell dissociation, synergism in isolating a single cell. Read More: https://www.purmabiologics...

What is Mem Composition, and how does it relate to cell culture?

Mem Composition refers to the specific formulation of Minimum Essential Medium (MEM), a widely used cell culture medium that provides essential nutrients, vitamins, amino acids, and minerals required for optimal cell growth. The MEM composition varies based on modifications such as the inclusion of non-essential amino acids,
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What is the difference between a hepatocyte and a Kupffer's cell?

Hepatocytes and Kupffer cells are two distinct types of liver cells, each playing a unique role in liver function.Hepatocytes: Hepatocytes are the primary functional cells of the liver, responsible for metabolism, detoxification, and protein synthesis. They play a crucial role in bile production, lipid metabolism, and glycogen storage.
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Improving Cell Culture Accuracy with Charcoal Stripped Serum

In cell culture research, precision is important, and the quality of the serum can greatly influence implications. The traditional use of fetal bovine serum (FBS) has been the standard in the industry, but this use is changing as charcoal-stripped fetal bovine serum (FBS) is seen for its capability to clean up the results by eliminating unwanted elements. Read More: https://sharing.clickup.co...

Why Is F-12 Medium a Preferred Choice for Hybridoma Cells?

Hybridoma culture is vital for producing monoclonal antibodies, where the growth medium choice influences cell viability, proliferation, and productivity. Among the media preferred, F 12 Medium (Ham's F-12 Medium) possesses a well-restored functional form, which favors the growth and production of high titers of antibodies. This medium is used by researchers and professionals dealing with biopharmaceuticals to ensure the proliferation of hybridoma cells, Read More: https://ext-6700630.livejo...

The Role of DEPC Water in RNA Preservation during Cell Culture

RNA preservation is central to the field of molecular biology. RNA is inherently fragile and subject to excessive degradation, making RNA preservation a difficult but critical aspect. From among RNA preservation aids during cell culture and other activities performed with RNA, DEPC-treated water. DEPC water has been extremely beneficial in preventing RNA deterioration, thereby securing validation of their findings. Read More: https://livepositively.com...

Applications of Hanks Solution in In Vitro Drug Testing

Hank's Balanced Solution (HBSS) has been in use for cell culture and testing drugs in vitro for quite some time now. This adaptable buffer solution is vital in order to provide the right environment for cell culture and to bring about accurate and repeatable drug testing outcomes. But why exactly is HBSS Solution used, and in what ways can it be applied in drug testing in vitro? Let's go and dive in. Read More: https://www.storeboard.com...

Horse Serum for Maintaining Long-Term Cultures in Labs

Long-term maintenance of cultures in the laboratory requires great care and the appropriate supplies. Horse serum is one of the serums considered pivotal in cell culture success. However, what makes horse serum an efficient product, and how does it differ from other serums such as fetal bovine serum (FBS)?Horse serum is the serum obtained from the blood of healthy horses and it is rich in growth factors, Read More: https://www.zupyak.com/p/4...

Choosing IMDM for Advanced Disease Modeling

In advanced disease modeling, the choice of a cell culture medium can play a significant role in providing the correct results. For many researchers, IMDM, or Iscove’s Modified Dulbecco's Medium, is preferred. What reasons may lead to the choice of IMDM over the others?IMDM is a highly enriched cell culture medium that provides all necessary cell growth and maintenance factors, including essential nutrients, Read More: https://purmabiologicsllc....